Ovarian Cancer: Tumor-Specific Urinary Micro-Peptides Profiling as Potential Biomarkers for Early Diagnosis
Ovarian Cancer: Tumor-Specific Urinary Micro-Peptides Profiling as Potential Biomarkers for Early Diagnosis
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Date
2020-10-29
Authors
Sulafa S. Murgan
Faisal J. Abd Elaziz
Abubakr M. A. Nasr
Mona E. E. Elfaki
Eltahir A. G. Khalil
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Volume Title
Publisher
MDPI
Abstract
Ovarian cancer is the second major lethal gynecologic malignancy in developing countries.
This study aimed to characterize urinary micro-peptides as potential diagnostic biomarkers for
ovarian cancer. In a prospective, longitudinal and case-controlled study and following informed
consent, urine and plasma samples were collected from 112 women with histologically-proven ovarian
cancer and 200 apparently healthy age-matched volunteers. Urinary micro-peptides were detected
and sequenced using SDS-PAGE and Edman degradation technique. Serum CA125 was detected in
less than a quarter (23.2%, 26/112) of patients. One or more urinary micro-peptides were detected
in about two thirds of the patients (62.5%, 70/112). A total of 40 patients had three bands (57.1%,
40/70), while two bands (15 and 35 kDa) were detected in 28.6% (20/70) of the patients. Isolated
45 kDa band was seen in 14.3% (10/70). No urinary micro-peptide was detected in the volunteers.
The 15 and 35 kDa bands disappeared after 6 months of regular chemotherapy, while the 45 kDa
band persisted in 2.9% (2/70) of the patients after treatment. The micro-peptides were identified as:
Catalase (45 kDa), -1 Acid Glycoprotein (35 kDa) and Peroxiredoxin-2 (15 kDa). Urinary catalase, -1
Acid Glycoprotein and Peroxiredoxin-2 can be useful biomarkers for early detection and treatment
response of ovarian cancer.
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Citation
Murgan, S.S., Abd Elaziz, F.J., Nasr, A.M., Elfaki, M.E. and Khalil, E.A., 2020. Ovarian Cancer: Tumor-Specific Urinary Micro-Peptides Profiling as Potential Biomarkers for Early Diagnosis. Proteomes, 8(4), p.32.