Molecular Detection of Aminoglycoside Resistance Gene arm A, ant, Acc3, Acc6 and Aph of Klebsiella pneumoniae isolated from clinical specimensin Khartoum State-Sudan 2022

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AlfatihAlamin Taha
AlmojtabaAlfatih Ahmed Ibrahim
Suliaman Gorashie Suliaman Khalil
Omer Hassan Omer Ahmed
Mustafa Yousif Adam Abaker
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Napata College
Background: Klebsiella pneumoniae (K. pneumoniae) has become one of the most agents of healthcare-associated infection in medical centers with the potential to cause several clinical infections including pneumonia, septicemia, and urinary tract and soft tissue infections. In recent years, the emergence of several types of resistance to multiple classes of antibiotics has caused critical clinical concern, leading to serious limitations in the treatment of these pathogens with subsequent increased morbidity and mortality. The objective of this study was the detection of a resistance gene in k.pneumoniae isolate from different types of samples in hospitals (Alia Specialist, Yastabshroun, Military hospital, Shargalneel, Omdurman for maternity) in Khartoum state – Sudan. A total of 50k.pneumoniae was isolated from different hospitals in Khartoum state. Objectives: Detection of Aminoglycoside Resistance Gene (arm A, ant, Acc3, Acc6, and Aph) in Klebsiella pneumoniae. Method: the cross-sectional analytical study was conducted in Khartoum state Sudan, A number of fifty samples were collected during the period from May to September 2022 and labeled culture these specimens in MacConkey agar in order to identify the bacteria perform gram staining technique and following biochemical tests (indole test, urease test, citrate utilization test, motility test, and growth on kligler iron agar) was performed and measure of antimicrobial sensitivity test by disc diffusion method (Kirby bour method), DNA extracted by use boiling method, and then perform multiplex PCR ( Ant andAcc3,Aph and Acc6 ) uniplex PCR (arm A). Result: Out of the 50 isolates from different samples including Urine, swabs, blood, sputum, and aspiration, most isolates from wound swabs 27(54%) male to female ratio were 0.6:1, patients' ages ranged from 1 year to 78 years with mean 53 (SD=22), the result of aminoglycoside resistance showed 28(56%), 34 (68%) and 37(74%) Gentamicin, Amikacin, and tobramycin respectively. Acc3 gene were detected in 21(42%) and 21 (42%) arm A, followed by 6 (12%) positive for Ant, and Acc6 was found in two isolates (4%), Aph was not detected there is not isolate share two or more genes. Conclusion: Our study findings confirmed the presence of aminoglycosides high resistance Klebsiella pneumoniae in Khartoum, Sudan with high prevalence of ant, armA, acc3 and acc6 genes especially acc3 and armA. The correlation between age group in the <1 year (40.0%) and acc6 gene and association between blood sample (40.0%) and acc6 gene.