Volume 2

Permanent URI for this community

http://dspace.napata.edu.sd/handle/123456789/339

Browse

Browsing Volume 2 by Author "Braah Mubarak Abdalraheem"

Now showing 1 - 1 of 1
  • Item
    Detection of Extended-Spectrum Beta-Lactamase and Carbapenemase Producing Gram Negative Bacteria Isolated from Intensive Care Unit -Khartoum-Sudan 2022
    (Napata College, 2023-12) Ahmed Emadeldin Osman; Alruomaisa Yousif Abker; Braah Mubarak Abdalraheem; Ezdehar Yousif Osman; Farida Farid Hamza; Saja Sulieman Nimir; Moil Babiker Alshiekh; Elrahman Fathelrahman Ahmed
    Background: Carbapenemase are enzymes that are a major cause of bacterial resistance to the Carbapenem family of antibiotics such as meropenem. ESBL are derived from genes for narrow-spectrum beta-lactamase by mutations that alter the amino acid configuration around the enzyme active sites. Objective: The study aimed to detect the production of carbapenemases and Extendedspectrum beta-lactamase among Gram-negative bacteria isolated from Intensive Care Units (Alselah Hospital, Ribat University Hospital, and Sharg Elnile Hospital in Khartoum state, Sudan.). Methodology: Descriptive cross-sectional facility-based study, conducted in Alselah Hospital, Ribat University Hospital, and Sharg Elnile Hospital in Khartoum state, Sudan, in which 100 gram negative bacteria were isolated from ICU (by simple random technique )in the period June to October 2022, and were investigated for susceptibility ,phenotypic detection of carbapenemases and ESBL. The isolated bacteria were purified by subculture in MacConkey ager, colonial morphology, gram stain and set of biochemical tests were done and antimicrobial susceptibility test was performed by Carbapenem antibiotic family. Finally the phenotypic detection of carbapenemases was done by modified Hodge test, and then used double disk diffusion method to detect extended spectrum beta lactamase. Result: One hundred isolates were investigated in this study, when susceptibility test was performed the highest susceptibility rate was registered for meropenem (84%) and the highest resistance rate for ceftriaxone (55%). ESBL producing bacteria were (16%) and Modified Hodge Test positive was (3%). Conclusion: The findings revealed that there was a minimum rate of ESBLs and Carbapenemase producing gram negatives isolated from ICU