Detection of Extended-Spectrum Beta-Lactamase and Carbapenemase Producing Gram Negative Bacteria Isolated from Intensive Care Unit -Khartoum-Sudan 2022
Detection of Extended-Spectrum Beta-Lactamase and Carbapenemase Producing Gram Negative Bacteria Isolated from Intensive Care Unit -Khartoum-Sudan 2022
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Date
2023-12
Authors
Ahmed Emadeldin Osman
Alruomaisa Yousif Abker
Braah Mubarak Abdalraheem
Ezdehar Yousif Osman
Farida Farid Hamza
Saja Sulieman Nimir
Moil Babiker Alshiekh
Elrahman Fathelrahman Ahmed
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Publisher
Napata College
Abstract
Background: Carbapenemase are enzymes that are a major cause of bacterial resistance
to the Carbapenem family of antibiotics such as meropenem. ESBL are derived from
genes for narrow-spectrum beta-lactamase by mutations that alter the amino acid
configuration around the enzyme active sites.
Objective: The study aimed to detect the production of carbapenemases and Extendedspectrum beta-lactamase among Gram-negative bacteria isolated from Intensive Care
Units (Alselah Hospital, Ribat University Hospital, and Sharg Elnile Hospital in
Khartoum state, Sudan.).
Methodology: Descriptive cross-sectional facility-based study, conducted in Alselah
Hospital, Ribat University Hospital, and Sharg Elnile Hospital in Khartoum state, Sudan,
in which 100 gram negative bacteria were isolated from ICU (by simple random technique
)in the period June to October 2022, and were investigated for susceptibility ,phenotypic
detection of carbapenemases and ESBL. The isolated bacteria were purified by subculture
in MacConkey ager, colonial morphology, gram stain and set of biochemical tests were
done and antimicrobial susceptibility test was performed by Carbapenem antibiotic
family. Finally the phenotypic detection of carbapenemases was done by modified Hodge test, and then used double disk diffusion method to detect extended spectrum beta
lactamase.
Result: One hundred isolates were investigated in this study, when susceptibility test was
performed the highest susceptibility rate was registered for meropenem (84%) and the
highest resistance rate for ceftriaxone (55%). ESBL producing bacteria were (16%) and
Modified Hodge Test positive was (3%).
Conclusion: The findings revealed that there was a minimum rate of ESBLs and
Carbapenemase producing gram negatives isolated from ICU
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