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Browsing Medical Sciences by Author "Abdelmonem Eltayeb Homida"

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    Evaluation of bio-insecticidal capacity of cannabis (Cannabis sativa L.) plants using GC-MS and phytochemical techniques
    (2022-03-25) Zahir Abbass Hilmi; Mutaman Ali A; Abdelmonem Eltayeb Homida; Yasir Mohamed Abdelrahim; Abdalla Ibrahim Abdalla Mohamed
    Plant-based pesticides are gaining attention as safe, effective, eco-friendly alternatives to synthetic pesticides. The aim of this study was to evaluate the bio-insecticidal capacity of Cannabis (Cannabis sativa) plants using GC-MS and phytochemical techniques and also mosquito's larvae as bioindicators. The phytochemical screening and the biological effect tests were run at Basic Sciences Laboratories, whereas GC-MS applications were run at the Central Laboratory, University of Gezira. The results showed that, Cannabis seeds contained flavonoids, alkaloids and steroids, and separated 5 spots through TLC. The GC-MS tests of Cannabis seeds detected Dronabinol (41.46%) as the main component, followed by Cannabinol (10.38%), Phytol (2.86%), Vitamin E (2.27%) and Caryophylene (2.07%). The ethanol extract of Cannabis seeds reflected LC50 of 218.1 mg/L, after 24. The more potent spot of Cannabis seeds was (3) against Anopheles larvae, but only spot (4) was more potent against Culex larvae and spot (5) against Aedes larvae. After one week of submission to Cannabis seed ethanol extract, the survived Anopheles larvae was 6.7%, while no survived Culex larvae, whereas 13.3% of Aedes larvae survived. Field assessment should be run to evaluate the sustainability of this products.
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    Evaluation of bio-insecticidal capacity of datura (Datura stramonium L.) leaves and flowers using GC-MS and phytochemical techniques
    (2022-04-04) Zahir Abbass Hilmi; Anwar Ali Ibrahim Mohamed; Mutaman Ali A Kehail; Abdelmonem Eltayeb Homida; Yasir Mohamed Abdelrahim
    The widespread use of synthetic insecticides has led to many negative consequences, resulting in increasing attention to natural products. The aim of this study was to evaluate the bio-insecticidal capacity of Datura stramonium leaves and flowers using GC-MS and phytochemical techniques and also mosquito's larvae as bioindicators. The phytochemical screening and the biological effect tests were run at Basic Sciences Laboratories, whereas GC-MS applications were run at the Central Laboratory, University of Gezira. The results showed that, Datura leaves contained saponnins, alkaloids, tannins but the flowers contained also flavonoids and steroids. 14 compounds were detected from Datura leaves ethanol extracts of which Butanol, 3-methyl is the main component (79.76%) followed by Toluene (6.14%) and Phytol (3.9%), while that of the flowers detected also 14 compounds of which formic acid, 3-methylbut-2-yl ester is the main component (82.22%). The ethanol extract of Datura leaves reflected LC50 of: 844.43 mg/L, against Cx. quiquefasciatus larvae, while that of the flowers was 636.62 mg/L. The ethanol extract of Datura leaves, after one week, left no survived Anopheles or Culex larvae, whereas, 10% of Aedes larvae were survived. The ethanol extract of Datura flowers, after one week, left no survived Anopheles or Culex or Aedes larvae, but some were developed to pupae. Field assessment should be run to evaluate the impact on the aquatic predators.