Aquaporin 5 promotes tumor migration and angiogenesis in non‑small cell lung cancer cell line H1299
Aquaporin 5 promotes tumor migration and angiogenesis in non‑small cell lung cancer cell line H1299
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Date
2020-01-07
Authors
Abdalkhalig Elkhider
Bing Wang
Xunli Ouyang
Mahmoud AL‑Azab
Williams Walana
Xiaotong Sun
Han Li
Yawei Tang
Jing Wei
Xia Li
Journal Title
Journal ISSN
Volume Title
Publisher
Elkhider
Abstract
Non‑small cell lung cancer (NSCLC) constitutes
the majority of all lung‑cancer cases. Aquaporin 5 (AQP5)
may be involved in NSCLC by promoting lung‑cancer initiation
and progression. The present study aimed to determine
the role of AQP5 in migration and angiogenesis using NSCLC
cells and HUVECs. AQPs 1, 3, 4, 5, 8 and 9 were screened in
the NSCLC cell line H1299, and the present results showed
that AQP5 mRNA was upregulated compared with the other
AQP genes. At the protein level, AQP5 was significantly
increased in H1299 cells compared with 16HBE cells. AQP5
knockdown in H1299 cells significantly decreased cell migration
compared with untransfected cells, as demonstrated by
both Transwell and wound closure assays. The present study
further investigated H1299 ability to promote HUVEC
vascularisation. The supernatants of both transfected and
untransfected H1299 cells were used as conditioned medium
for HUVECs, and tube formation was measured. The supernatant
of AQP5‑downregulated cells exhibited significantly
low tube formation potential compared with untransfected
cells. Similarly, vascular endothelial growth factor was significantly
increased in control cells (si‑NC) compared with cells
transfected with small interfering RNA targeting AQP5. The
present study found that AQP5 downregulation significantly
decreased the phosphorylation level of epidermal growth
factor receptor and the activity of the ERK1/2 pathway. In
summary, the present study suggested that AQP5 influenced
migration and angiogenesis in NSCLCs in vitro and may
potentially exhibit similar in vivo effects.
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Citation
Elkhider, A., Wang, B., Ouyang, X., Al‑Azab, M., Walana, W., Sun, X., Li, H., Tang, Y., Wei, J. and Li, X., 2020. Aquaporin 5 promotes tumor migration and angiogenesis in non‑small cell lung cancer cell line H1299. Oncology letters, 19(3), pp.1665-1672.