The Reference Ranges for Lymphocytes Subsets of Healthy Adults individuals by Immunophenotyping.
The Reference Ranges for Lymphocytes Subsets of Healthy Adults individuals by Immunophenotyping.
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Date
2014-03-08
Authors
Zahir A.Hilmi
Hussam M. Abdelltef
Ihsan M. Osman
Osama A. Altayeb
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Abstract
Introduction and aims: Immunophenotyping by flow cytometry is the most advance
and accurate technique for differentiation between lymphocyte sub populations of B
and T cells. This descriptive cross-sectional study was performed to establish the
normal reference ranges for several lymphocyte sub-populations of healthy adult
Sudanese: (CD3, CD4, CD8 and CD19). Materials and Methods: Peripheral blood
samples (n = 100) were collected from healthy adult Sudanese from Khartoum State,
Sudan. Their mean age was (31± 13) years ranged from (18 to 80) years, and their sex
ratio male to females was (1:1). The hematological parameters of them were analyzed
by hematology analyzer (Sysmex). The flow cytometry was used to determine
percentages and absolute count (cell/μl) of CD3, CD4, CD8 T cells and CD19 B cell.
SPSS version 16 was used for statistical analysis. Results: The percentages and mean
absolute count (cell/μl) of lymphocytes and lymphocyte sub-populations were obtained
as follows: lymphocytes (35.8% ± 7.3 ; 2120.1 ± 652), CD3 (58.9% ± 14.7 ; 1242 ±
481), CD4 (35.9 % ± 20.1% ; 761 ± 315), CD8 ( 18.1% ± 11.1% ; 425 ± 195),
CD19 (9.8% ± 5.4%; 214 ± 141). The CD4/CD8 ratio was found to be (1.98 ± 0.85).
Conclusion: there was no significant difference according to sex and age in different
CD values obtained.
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Abdelltef, H.M., Osman, I.M., Hilmi, Z.A. and Altayeb, O.A., 2014. The reference ranges for lymphocytes subsets of healthy adults individuals by immunophenotyping.