The Reference Ranges for Lymphocytes Subsets of Healthy Adults individuals by Immunophenotyping.

No Thumbnail Available
Zahir A.Hilmi
Hussam M. Abdelltef
Ihsan M. Osman
Osama A. Altayeb
Journal Title
Journal ISSN
Volume Title
Introduction and aims: Immunophenotyping by flow cytometry is the most advance and accurate technique for differentiation between lymphocyte sub populations of B and T cells. This descriptive cross-sectional study was performed to establish the normal reference ranges for several lymphocyte sub-populations of healthy adult Sudanese: (CD3, CD4, CD8 and CD19). Materials and Methods: Peripheral blood samples (n = 100) were collected from healthy adult Sudanese from Khartoum State, Sudan. Their mean age was (31± 13) years ranged from (18 to 80) years, and their sex ratio male to females was (1:1). The hematological parameters of them were analyzed by hematology analyzer (Sysmex). The flow cytometry was used to determine percentages and absolute count (cell/μl) of CD3, CD4, CD8 T cells and CD19 B cell. SPSS version 16 was used for statistical analysis. Results: The percentages and mean absolute count (cell/μl) of lymphocytes and lymphocyte sub-populations were obtained as follows: lymphocytes (35.8% ± 7.3 ; 2120.1 ± 652), CD3 (58.9% ± 14.7 ; 1242 ± 481), CD4 (35.9 % ± 20.1% ; 761 ± 315), CD8 ( 18.1% ± 11.1% ; 425 ± 195), CD19 (9.8% ± 5.4%; 214 ± 141). The CD4/CD8 ratio was found to be (1.98 ± 0.85). Conclusion: there was no significant difference according to sex and age in different CD values obtained.
Abdelltef, H.M., Osman, I.M., Hilmi, Z.A. and Altayeb, O.A., 2014. The reference ranges for lymphocytes subsets of healthy adults individuals by immunophenotyping.