Detection of Mucosal HPVs, HPV16 and HPV18 in Cervical Squamous Cell Carcinomas in Sudan

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Zahir A. Hilmi
Nusiaba H. Abdo
Bandar H. Aloufi
Suheir R. Rehan
F. Hamedelnil
M. Abdallatif
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Background: Human papillomavirus (HPV) is the principal etiology for cervical cancer. Few contradictory researches were published in Sudan. Objectives: This research aimed to explore the roles of HPV16 and HPV18 in cervical cancer in Sudan.Materials and Methods: The DNA was extracted from sections of paraffin embedded tissues of Sudanese women (n, 63) with oral squamous cell carcinomas and controls (n, 17). The patients DNA samples (100ng/μl) were amplified by mucosal HPV GP5+/GP6+, HPV16 type specific and HPV18 type specific primers. Results: GP primers detected mucosal HPVs in 75% (n, 47) of the patients, and none of the controls. HPV16 and HPV18 types were identified from 70% (n, 33) and 9% (n, 4) of the GP positives. HPV16 was identified from all tumor differentiations degrees specially moderately differentiated tumors, while HPV18 was identified from moderate and well differentiated tumors. HPV16 was isolated from all ages but mostly isolated from older patients, while HPV18 was isolated from younger patients. Ten unknown mucosal HPV types were detected. HPV16 and HPV18 were mostly detected in house wives ethnically from Central Sudan. Conclusion: HPV 16 is the most important determinant for development of cervical cancer in Sudan, with less contribution of HPV18. HPV screening and vaccination program has to be established in young Sudanese women.
Hilmi, Z.A., Abdo, N.H., Aloufi, B.H., Rehan, S.R., Hamedelnil, Y. and Abdallatif, H., 2014. Detection of Mucosal HPVs, HPV16 and HPV18 in Cervical Squamous Cell Carcinomas in Sudan.