Detection of Mucosal HPVs, HPV16 and HPV18 in Cervical Squamous Cell Carcinomas in Sudan
Detection of Mucosal HPVs, HPV16 and HPV18 in Cervical Squamous Cell Carcinomas in Sudan
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Date
2014-08-29
Authors
Zahir A. Hilmi
Nusiaba H. Abdo
Bandar H. Aloufi
Suheir R. Rehan
Yousif
F. Hamedelnil
Husam
M. Abdallatif
Journal Title
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Volume Title
Publisher
AENSI
Abstract
Background: Human papillomavirus (HPV) is the principal etiology for cervical
cancer. Few contradictory researches were published in Sudan. Objectives: This
research aimed to explore the roles of HPV16 and HPV18 in cervical cancer in
Sudan.Materials and Methods: The DNA was extracted from sections of paraffin
embedded tissues of Sudanese women (n, 63) with oral squamous cell carcinomas
and controls (n, 17). The patients DNA samples (100ng/μl) were amplified by mucosal
HPV GP5+/GP6+, HPV16 type specific and HPV18 type specific primers. Results:
GP primers detected mucosal HPVs in 75% (n, 47) of the patients, and none of the
controls. HPV16 and HPV18 types were identified from 70% (n, 33) and 9% (n, 4) of
the GP positives. HPV16 was identified from all tumor differentiations degrees
specially moderately differentiated tumors, while HPV18 was identified from moderate
and well differentiated tumors. HPV16 was isolated from all ages but mostly isolated
from older patients, while HPV18 was isolated from younger patients. Ten unknown
mucosal HPV types were detected. HPV16 and HPV18 were mostly detected in house
wives ethnically from Central Sudan. Conclusion: HPV 16 is the most important
determinant for development of cervical cancer in Sudan, with less contribution of
HPV18. HPV screening and vaccination program has to be established in young
Sudanese women.
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Citation
Hilmi, Z.A., Abdo, N.H., Aloufi, B.H., Rehan, S.R., Hamedelnil, Y. and Abdallatif, H., 2014. Detection of Mucosal HPVs, HPV16 and HPV18 in Cervical Squamous Cell Carcinomas in Sudan.