IL-8 analogue CXCL8 (3-72) K11R/G31P, modulates LPS-induced inflammation via AKT1-NF-kβ and ERK1/2-AP-1 pathways in THP-1 monocytes

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dc.contributor.authorWilliams Walana
dc.contributor.authorJing-Jing Wanga
dc.contributor.authorIddrisu Baba Yabasinb
dc.contributor.authorMichael Ntim
dc.contributor.authorSylvanus Kampo
dc.contributor.authorMahmoud Al-Azab
dc.contributor.authorAbdalkhalig Elkhider
dc.contributor.authorEugene Dogkotenge Kuugbee
dc.contributor.authorJya-wei Cheng
dc.contributor.authorJohn R. Gordon
dc.contributor.authorFang Li
dc.date.accessioned2022-12-29T09:28:34Z
dc.date.available2022-12-29T09:28:34Z
dc.date.issued2018-08-17
dc.description.abstractIL-8 is elevated during inflammation, and it initiates cascade of down-stream reactions. Its antagonist, CXCL8 (3–72) K11R/G31P (G31P), represses inflammatory reactions via competitive binding to CXC chemokine family, preferentially G protein-couple receptors (GPCRs) CXCR1/2. This study reports the effect of G31P on the transcription profile of lipopolysaccharide (LPS) induced inflammation in THP-1 monocytes ex-vivo. LPS (1 μg/ ml) induced elevation of IL-8 was significantly reduced by G31P (20 μg/ml and 30 μg/ml), with relatively increased inhibition of CXCR2 than CXCR1. Transcription of IL-1β, IL-6, and TNF-α were significantly inhibited, while IL-10 remained relatively unchanged. G31P treatment also had repressing effect on the inflammatory associated enzymes COX-2, MMP-2, and MMP-9. Significant restriction of c-Fos, and NF-kβ mRNA expression was observed, while that of c-Jun was marginally elevated. Conversely, SP-1 mRNA expression was seen to increase appreciably by G31P treatment. While the translation of pAKT, pERK1/2, and p65- NF-kβ were downregulated by the G31P following THP-1 cells stimulation with LPS, reactive oxygen species (ROS) expression was on the positive trajectory. Collectively, the IL-8 analogue, G31P, modulates the inflammatory profile of LPS induced inflammation in THP-1 monocytes via AKT1-NF-kβ and ERK1/2-AP-1 pathways.
dc.identifier.urihttp://dspace.napata.edu.sd/handle/123456789/173
dc.language.isoen
dc.publisherElsevier
dc.titleIL-8 analogue CXCL8 (3-72) K11R/G31P, modulates LPS-induced inflammation via AKT1-NF-kβ and ERK1/2-AP-1 pathways in THP-1 monocytes
dc.typeArticle
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