Evaluation of Polymerase Chain Reaction for Direct Detection of Escherichia coli Strains in Environmental Samples

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dc.contributor.authorSafa A. Sherfi
dc.contributor.authorHamid A. Dirar
dc.contributor.authorBadr E. Hago
dc.contributor.authorMohamed E. Ahmed
dc.contributor.authorHassan A. Musa
dc.contributor.authorHassan Abu Aisha
dc.contributor.authorImadeldin E. Aradaib
dc.date.accessioned2023-01-09T12:26:06Z
dc.date.available2023-01-09T12:26:06Z
dc.date.issued2007
dc.description.abstractThe potential of the Polymerase Chain Reaction (PCR), as a means of detecting Escherichia coli (E. col’) DNA in suspected environmental samples, was studied. Using a pair of outer primers Pl and P2, selected from wdA gene, which encodes £. coli glucuronidase, the PCR-based assay resulted in amplification of a 486 base pair (bp) PCR product. £. cofi strains from different environmental sources including recycled and drinking water as well as stagnant water were detected by this nested PCR-based assay. Amplification products were visualized on ethidium bromide-stained agarose gel. The sensitivity of the PCR assay was 100 fg of bacterial DNA with ethidium bromide-stained agarose gels. Using a pair of internal (nested) primers P3 and P4, the nested PCR produced a 186 bp PCR product. The nested PCR increased the sensitivity of the PCR assay by 1,000 times and specific PCR products were detected from 0.1 fg of bacterial DNA. Amplification product was not detected when the nested PCR-based assay was applied to DNA from other related bacteria including, Salmonella, Pseudomonas and Proteus or nucleic acid-free water. Application of this nested PCR-based assay to environmental samples resulted in direct detection of Z. coli DNA from sewage water, tap water, drinking water at Shambat Campus, University of Khartoum, Sudan. This nested PCR-based assay should provide a rapid, sensitive and specific assay for direct detection and quantification of £. cofi in environmental samples suspected to contain the organism.
dc.identifier.citationAradaib, I.E., 2008. Evaluation of Polymerase Chain Reaction for Direct Detection of Escherichia coli Strains in Environmental Samples.
dc.identifier.issn18164935
dc.identifier.urihttp://dspace.napata.edu.sd/handle/123456789/185
dc.language.isoen
dc.publisherAcademic Journals
dc.titleEvaluation of Polymerase Chain Reaction for Direct Detection of Escherichia coli Strains in Environmental Samples
dc.typeArticle
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