Identification of Common Aerobic Bacteria Associated With Type 2 Diabetic Patients in Khartoum State

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Amal Yassin Al-Tahir Al-Hussein
Salawat Ahmed Noah Ahmed
Fatima Shames Aldeen Ali Suleiman
Maria Musa Abdullah Hussein
Yousra El-Tayeb Ahmed Saad
Yusriya Muhammed Al-Siddiq al-Barr
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Napata College
IV Abstract Background: Diabetic foot ulcers is among the most common complications of diabetes mellitus which significantly causes hospitalization and is the most prevalent etiology of non-traumatic amputation worldwide. Knowledge of the microbial burden in the ulcers and antibiotics susceptibility pattern may improve patients’ care and management. this study was aim to isolate, identify and carry out antibiotic susceptibility testing on bacterial isolates associated with foot ulcers among type 2 diabetic mellitus. Method: A cross-sectional laboratory based study was conducted in selected Hospitals in Khartoum swabs samples were collected before dressing from patients suffering diabetic foot ulcers attended diabetic center and surgical department during the study period .Data were collected using structured questionnaire. The clinical isolates were purified by streaking on suitable selective and differential culture media. They were identified on the basis of the results of microscopically examinations, Gram reactions, cultural characters, biochemical tests and antibiotic susceptibility testing was done according to clinical and laboratory standard institutes guidelines. Result: A total of Ninety two pathogenic bacteria were isolated from patients with a diabetic foot ulcer. The most prevalent detected bacteria were Staphylococcus .aureus( 34%) , Klebsiella sp. (10.9%), Proteus.mirabilis (27.2%) ,Proteus.vulgaris (12%) ,pseudomon.aeruginosa (17.4%).Gram-positive (Staphylococcus .aureus) isolates were susceptible to Gentamycin, Vancomycin and Imepenm Gram-negative isolates were also sensitive to Imepenem and Ciprofloxacin The most resistant antibiotic was Amoxacillin Conclusion : The pathogens causing diabetic foot ulcers show vary in sensitivity and resistant to many of the routinely used medications. However, resistance is being developed to some of the antibiotics such as Ceftrixone Therefore, the culture of the specimen to identify the causative agent and adequate knowledge of the susceptibility pattern are critical for the appropriate management of diabetic foot ulcers.