Phytochemicals Composition And Antimicrobial activity, Of Allium Satvium (Clove) [garic]

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Hind Alhady Omer Mohammed
MontasirAbdAlbasit Hassan Ahamed
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Napata College
Allium sativum L. belongs to family Liliaceae, has been used as a medicine through ancient and modern time to prevent and treat a wide range of conditions, diseases and tumors due to presence of phenolic compounds and volatile oils which contains sulfur atom in their chemical structures ,and the major antibiotic ALLICIN(unstable) which proven to has an antimicrobial effect against the pathogenic bacteria,parasites, viruses and fungi. This study was carried out to determine the phytochemical composition of the macerated ethanolic extract of the garlic pulp using GC-MS technique and to evaluate its antimicrobial activity against pathogenic bacterial strains usig disc diffusion method,. GC-MS revealed a total of (16)… compounds which were identified in the ethanolic extract ranging from terpenes, essential oils, , phenolic compounds, esters, and others. The major constituents were; Diallyldisulphide (14.26%) 17.34 %) ,1,2,3- thiadiazole,5-methyl- (4.24%, 5.91%) and Trisulfide,di-2-propnyl (19.84%, 14.36% ) respectively. Then the disc diffusion methods was employed to explore the antimicrobial activity of ethanolic extract against four pathogenic bacterial strains S. aureus ,B.subtillis , P. aeruginosa , E. coli , C . Albican, The zone of inhibition observed was 10.5 – 13.5 mm at higher Concentration for used .