Cardiac myosin heavy chains in mice treated with NG-nitro-L -arginine methyl ester and thyroxine
Cardiac myosin heavy chains in mice treated with NG-nitro-L -arginine methyl ester and thyroxine
No Thumbnail Available
Date
2013-11-15
Authors
Mukhallad A. M. Mohammad
Muhanad S. Abdelwahab
Mohamad M. J. Mohamad
Othman El shboul
Waleed R. Ezzat
Journal Title
Journal ISSN
Volume Title
Publisher
Springer Verlag
Abstract
Abstract The aim of this study was to evaluate the effect of
NG-nitro-L-arginine methyl ester and thyroxine on the distribution
of cardiac myosin heavy chains in mice. Myocardial
hypertrophy was induced in mice by intraperitoneal injection
with L-thyroxine and oral administration of NG-nitro-L-arginine
methyl ester (L-NAME). Mice were randomly allocated
into five groups: control, high and low doses of thyroxine and
L-NAME. Heart weight divided by body weight was calculated
and used as indicator for cardiac hypertrophy. Myosin
heavy chains (MHCs) were separated using 4 % polyacrylamide
gel electrophoresis. Hypertrophy was induced in mice
treated with thyroxine and a high dose of L-NAME and was
accompanied by a shift toward -MHC in thyroxine-treated
mice and -MHC in L-NAME-treated mice. There was no
difference with respect to MHC between a low dose of LNAME
and a high dose; however, low doses of L-NAME did
not result in cardiac hypertrophy. In conclusion, L-NAME
treatment changes the MHC distribution from - toward -
MHC and this transition in the MHCs occurs before heart
hypertrophy. Further investigations are needed to determine
whether L-NAME treatment causes this transition via a direct
or indirect effect on the heart muscles.