Batch1

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Browsing Batch1 by Author "Muzdalifah Abdellah Abdrazig"

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    PHYTOCHEMICAL COMPOSITION, ANTIOXADANT a n d ANTIMICROBIAL OF Calotropis procera (AIT.) LEAVES
    (Napata College, 2021) Omer Bashir Khlefa; Shaza Eltayeb Mohammed; Muzdalifah Abdellah Abdrazig
    The world is fertile with natural and medicinal plants, which are now more focused than ever, because they have the capability of producing many benefits to society, indeed to mankind, especially in the line of medicine and pharmacology. The medicinal power of these plants lie in phytochemical constituents that cause definite pharmacological actions on the human body. Calotropis procera (Ait.) leaves, from Asclepiadaceae family is a medicinal plant, which has been used for centuries as a traditional medicinal plant in different countries. This study aimed to the determination of chemical composition and evaluation of two pharmacological activities; antioxidant, and antimicrobial. Macerated methanolic extract was prepared.and then Gas Chromatography-Mass Spectroscopy profiling was done.GC-MS revealed a total of eleven compounds which were identified in the methanolic extract ranging from fatty acids and alkane compounds. The major constituents were– Phytol (26.60%), Tridecanoic acid (22.07%), and 9-Hexadecenoic acid(17.50%). The antioxidant properties of the methanolic extract was screened using DPPH assay, and compared with Propyl gallate as standard. Methanolic extract showed significant scavenging activity (46±0.00), but revealed percentage inhibition lower than that obtained by Propyl gallate (92±0.01). The disc diffusion method was employed to explore the antimicrobial activity of methanolic leaf extract agamicrobs ,the extract proved to be an efficient antimicrobial agent. The highest zone of inhibition observed was 19 mm obtained by P.aeruginosa ,whereas the less effective was against B.subtillis (12mm)at the concentration 10%,with the Staandard deviation(1.414) The important role that C.procera plants play in folk medicine has led us to develop this study in order to investigate the major pharmacological activities, so this plant can be used in future as a valuable source of pharmacologically active antioxidants, antimicrobial agents.